Journal:
Article Title: Reverse cholesterol transport is regulated by varying fatty acyl chain saturation and sphingomyelin content in reconstituted high density lipoproteins
doi: 10.1016/j.metabol.2006.09.021
Figure Lengend Snippet: Each indicated rHDL species (approximately 1 μg protein) was subjected to electrophoresis on a 8–25% polyacrylamide native gel. Protein bands were revealed by coomassie staining. Particle size markers were determined by densitometry analysis using a Fluorchem8800 gel analyzer (Alpha Innotech Corp. San Leandro, CA) loaded with AlphaEasyFC software for calculations. A. Representative gel for rHDL species containing DOPC. In order to minimize the graph’s complexity, rHDL composition is displayed as molar percentage of the varying PC species. Thus, 100% DSPC, DSPC only; 25% DOPC, DSPC:DOPC (187.5:62.5); 50% DOPC, DSPC:DOPC (75:75); and 75% DOPC, DSPC:DOPC (62.5:187.5). B: Representative gel for rHDL species containing DLOPC and SM. rHDL species’ composition is displayed according to SM content only. Thus, 100:0 corresponds to DSPC:DOPC:SM (187.5:52.5:0), 80:20 to DSPC:DOPC:SM (150:50:50), 60:40 to DSPC:DOPC:SM (112.5:37.5:100), and 20:80 to DSPC:DOPC:SM (37.5:12.5:200). Data from densitometry measurements of the rHDL species’ particle size are reported in Tables 3A and 3B. Particles size markers were bovine serum albumin (7.1 nm), lactate dehydrogenase (8.2 nm), thyroglobulin (9.2 nm), catalase (12.2 nm), and apoferitin (17.0).
Article Snippet: Particle size markers were determined by densitometry analysis using a Fluorchem8800 gel analyzer (Alpha Innotech Corp. San Leandro, CA) loaded with AlphaEasyFC software for calculations.
Techniques: Electrophoresis, Staining, Software
